Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects.

Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.
It also includes information pertinent to the sequence(s), including length and molecular weight. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. Proteomes. X-Ray Induced Mutant Strains of Escherichia Coli. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. Aminoglycosides are useful primarily in infections involving aerobic, Gram-negative bacteria, such as Pseudomonas, Acinetobacter, and Enterobacter. Gene ID: 13982014, updated on 16-Dec-2016. A second streptomycin resistance gene from Streptomyces griseus codes for streptomycin-3"-phosphotransferase. Streptomycin resistance produces an absolute requirement for polyamines for growth of an Escherichia coli strain unable to synthesize putrescine and spermidine [delta(speA-speB) delta specC] . Sequence clusters. UniProtKB. have the same checksum value, the likelihood that this would happen S.griseus streptomycin-3'-phosphotransferase gene, complete cds. AU - Rudi, Knut. The phage facilitates microscopic fluorescence detection due to the high expression of green fluorescence protein which also simplifies the operative protocol as well. We have applied the streptomycin resistance marker as a positive selection marker to perform homologous recombination through conjugation and successfully generated a double unmarked in-frame targeted mutant 1401D88 tfox arcA. Protein sets from fully sequenced genomes.

More...

,

This subsection of the Names and taxonomy section indicates if the gene coding for the protein originates from the hydrogenosome, the mitochondrion, the nucleomorph, different plastids or a plasmid. Proteomes. from the sequence. Automatic assertion inferred from database entriesi, Automatic assertion inferred from database entriesi, The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms, Integrated resource of protein families, domains and functional sites, Superfamily database of structural and functional annotation. UniRef. It was previously known that streptomycin resistance came from mutations in the rpsL (ribosomal protein L) gene, as streptomycin's mode of action is to bind to this protein and restrict ribosome function. b Determines streptomycin dependence (100 ~g/ml). Streptomycin is used to study mechanisms of streptomycin resistance and is often used together with penicillin and other agents to inhibit bacterial contamination in cell culture applications. Help. Protein sets from fully sequenced genomes. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. et al., Travis, R.M. It is given by injection into a vein or muscle. of multiple genes (paralogs).

More...

,

When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later.

More...

,

This indicates the type of evidence that supports the existence of the protein.

This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

More...

Sequence statusi: Complete. MILLER CP, BOHNHOFF M. The development of bacterial resistance to chemotherapeutic agents. AU - Ludvigsen, Jane. Annotation systems. The principal side-effects of aminoglycosides are ototoxicity and nephrotoxicity. patient 1 (DNA sample 1) Which of the following best describes the mechanism of action of the antibiotic streptomycin? Streptomycin (STR)-resistant M. tuberculosis clinical isolates have been analyzed to look for mutations in the rpsL , rrs , and gidB genes. Turnock, G. : The action of streptomycin in a mutant of Escherichia coli with increased sensi- tivity to the antibiotic. Help. It works by blocking the ability of 30S ribosomal subunits to make proteins, which results in bacterial death. Looking at your data collected from the gel electrophoresis, which patient do you think has the least ability to resist illness?

The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) Molecular Microbiology (1993) 9(6), 1239-1246 Molecular basis of streptomycin resistance in Mycobacterium tuberculosis: alterations of the ribosomal protein S12 gene and point mutations within a functional 16S ribosomal RNA pseudoknot Marion Finken, Philip Kirschner, Albrecht Meier, the 16S and/or 23S rRNA from chemical probes when Annette Wrede and Erik C. Bottger' they bind to … You are using a version of browser that may not display all the features of this website. using the generator polynomial: x64 + x4 + x3 + x + 1. We have applied the streptomycin resistance marker as a positive selection marker to perform homologous recombination through conjugation and successfully generated a double unmarked in-frame targeted mutant 1401D88 tfox arcA. UniParc. Stay Connected to Science. By growing an aliquot of E. coli on TBAB + Strep plates, we observed a small number of colonies. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) Protein knowledgebase. Streptomycin acts on the ribosome, inhibiting the translation of mRNA and thereby disrupting protein synthesis. Mutations conferring resistance to low levels of kanamycin in Escherichia coli have been mapped at 3 locations: the unc locus (min. … of multiple genes (paralogs).

The resistance gene in pENTR223.1 is the aminoglycoside adenylyltransferase gene aadA (streptomycin 3''(9)-O-nucleotidyl transferase; aminoglycoside 3"-adenylyltransferase (AAD(3”)(9); ANT(3”)(9)), which confers resistance to both spectinomycin and streptomycin.

However UniProtKB may contain entries with identical sequences in case Protein knowledgebase. Annotation systems. Y1 - 2018/3/8 . Heinzel P(1), Werbitzky O, Distler J, Piepersberg W. Author information: (1)Institut für Biochemie der Technischen Hochschule Darmstadt, Federal Republic of Germany. Required for streptomycin resistance (PubMed:3357770). Die aerob lebenden Streptomyceten gehören zu der großen Gruppe der Aktinobakterien.

More...

,

This subsection of the Names and taxonomy section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. We demonstrate that streptomycin resistance is associated with mutations implicated in ribosomal resistance. Combined with a natural transformation-based knockout system and this genetic technique, multiple deletion mutants or attenuated strains of H. parasuis can … Protein sets from fully sequenced genomes. UniParc. We'd like to inform you that we have updated our Privacy Notice to comply enterica serovar Heidelberg, Salmonella enterica subsp. Specifically Streptomycin binds to four nucleotides of 16S rRNA and a single amino acid of protein S12. The current subsections and their content are listed below:

More...

,

This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

More...

,

The checksum is a form of redundancy check that is calculated These are stable identifiers and should be used to cite UniProtKB entries. J Bacteriol. Streptomycin, the first antibiotic used in tuberculosis control programs, perturbs protein synthesis at the ribosome level. The Mycobacterium tuberculosis pandemic is a major health problem, further complicated by an increasing incidence of drug-resistant isolates and the existence of highly transmissible strains, such as those in the Beijing family. As a result, this agent interferes with the assembly of initiation complex between mRNA and the bacterial ribosome, thereby inhibiting the initiation of protein synthesis. Protein knowledgebase. The mutations found either lead to amino acid changes in ribosomal protein SI2 or alter the primary structure of the 16S rRNA. For active tuberculosis it is often given together with isoniazid, rifampicin, and pyrazinamide. In the case of antibiotic resistance, the mutation rate is frequently defined as the in vitro frequency at which detectable mutants arise in a bacterial population in the presence of a given antibiotic concentration.1 Streptomycin, the protein-inhibiting 16S rRNA subunit binding aminoglycoside, interferes with the binding of the formyl- methionyl-tRNA to the 30S subunit. Please consider upgrading,

An evidence describes the source of an annotation, e.g. Save to my folders. The integron was located on a plasmid and … an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.

Adenylates streptomycin on the O-6 residue (By similarity). Protein sets from fully sequenced genomes. Escherichia coli Ouellette, M. et al., Clark, N.C. et al., Shaw, K.J. We demonstrate that streptomycin resistance is associated with mutations implicated in ribosomal resistance. As shown in Fig. Il appartient à la classe des aminosides (aminoglycosides). Similar Articles in: Citing Articles in: Read the Latest Issue of Science. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) Please consider upgrading,

An evidence describes the source of an annotation, e.g. Biochem J. Sequence clusters. Facebook; Twitter; Related Content . Ribosomal proteins from chloroplasts of Nicotiana tabacum L. (cv.

This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

More...

Sequence statusi: Complete. Systems used to automatically annotate proteins with high accuracy: UniRule (Expertly curated rules) Protein sets from fully sequenced genomes. pS194 is a naturally occurring Staphylococcus aureus plasmid encoding streptomycin resistance. Streptomycin is an aminoglycoside. Automatic assertion inferred from database entriesi, Automatic assertion inferred from database entriesi, Integrated resource of protein families, domains and functional sites, Superfamily database of structural and functional annotation. In this mutant, a single nucleotide transition, C » T, in the chloroplast gene for ribosomal protein S12 alters codon 90 from proline to serine while the nucleotide sequence of the chloroplast 16 S rRNA gene is identical to that of the wild type. Citing Literature. Verhoef, C., Haan, P. G. de: Genetic recombination in Escheriehia coli. Streptomycin is an aminoglycoside antibiotic that works by binding to the bacterial 30S ribosomal subunit, causing misreading of t-RNA, leaving the bacterium unable to synthesize proteins vital to its growth. Sequence archive. This includes tuberculosis, Mycobacterium avium complex, endocarditis, brucellosis, Burkholderia infection, plague, tularemia, and rat bite fever. Catherine Salles , Catherine Salles Molecular Genetics and Microbiology, CNRS-UPR9007, Université Paul Sabatier. Streptomycin (STR) resistance in Mycobacterium, a genus that includes important human pathogens, such as Mycobacterium tuberculosis, along with fast-growing opportunistic species, such as Mycobacterium fortuitum, is due to several mechanisms. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.

More...

,

A UniProt proteome can consist of several components.

The component name refers to the genomic component encoding a set of proteins.

More...

,

This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.

More...

,

This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. Resistance to streptomycin can occur by this mechanism since this agent binds to a single site on the 30S subunit of the ribosome. Some mutations could cause a strongly hyperaccurate phenotype and lead to streptomycin dependence . Protein knowledgebase. UniParc. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.

More...

, Automatic assertion inferred from database entries,

This subsection of the Names and taxonomy section provides information on the name(s) of the organism that is the source of the protein sequence.

More...

,

This subsection of the Names and taxonomy section shows the unique identifier assigned by the NCBI to the source organism of the protein.

However UniProtKB may contain entries with identical sequences in case Chromosomally acquired streptomycin resistance is frequently due to mutations in the gene encoding the ribosomal protein S12, rpsL. Annotation systems. Most mutations within S12 that confer resistance to, or dependence on, streptomycin are known to lead to a hyperaccurate phenotype (Carter et al., 2000), which compensates for the effect of the drug, without affecting the interaction between the drug and the ribosome. 118, 659-666 (1970). When strA and strB were cloned separately on a lacIq/Ptac-based expression vector in Escherichia coli, only the protein encoded by strA was produced. AU - Amdam, Gro. Resistance to the other aminoglycosides by this mechanism is uncommon since they bind to multiple sites on both ribosomal subunits and high-level resistance cannot be selected by a single step. In our previous study, we have improved ε-PL production by S. albulus M-Z18 through iterative introduction of streptomycin resistance. Sie produzieren auch Duftstoffe, die dem frischen Waldboden seinen charakteristischen Geruch verleihen. The streptomycin-dependent and target gene mutations [2], while 16S rRNA methylases, which mutants need streptomycin to keep their survival because their have recently emerged, confer high-level resistance to all clinically ribosomal proteins require the binding of streptomycin for available aminoglycosides except streptomycin [3–5]. The streptomycin resistance (StrR) mutations K42N and P90S in ribosomal protein S12 impair growth on rich medium. 83), a locus we have designated kanA (MIN. 6 Mutations associated with streptomycin resistance in Mycobacterium tuberculosis have been identified in the rpsL and rrs genes, which encode the ribosomal protein S12 and 16S rRNA, respectively. UniParc.

Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.
DNA Data Bank of Japan; a nucleotide sequence database, Ensembl bacterial and archaeal genome annotation project, KEGG: Kyoto Encyclopedia of Genes and Genomes, Pathosystems Resource Integration Center (PATRIC), Database of comparative protein structure models, ProtoNet; Automatic hierarchical classification of proteins, MobiDB: a database of protein disorder and mobility annotations. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.

More...

,

This subsection of the 'Entry information' section provides one or more accession number(s). Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.

More...

, The European Molecular Biology Laboratory, State Secretariat for Education, Research and Innovation, phosphotransferase activity, alcohol group as acceptor, Aminoglycoside O-phosphotransferase APH(6)-Ic, Salmonella enterica subsp. 7, 8 More than half of the streptomycin-resistant clinical isolates present … Streptomycin irreversibly binds to the 16S rRNA and S12 protein within the bacterial 30S ribosomal subunit. This is known as the 'taxonomic identifier' or 'taxid'.

More...

,

This subsection of the Names and taxonomy section contains the taxonomic hierarchical classification lineage of the source organism. Sequence clusters. Streptomycin resistance of the SRl mutant therefore seems to be the consequence of a mutation in the chloroplast DNA coding for a chloroplast ribosomal protein. Sequence archive. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.

More...

,

This section provides any useful information about the protein, mostly biological knowledge.

More...

,

The Gene Ontology (GO) project provides a set of hierarchical controlled vocabulary split into 3 categories:

More...

,

This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.

More...

,

This subsection of the Names and taxonomy section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.

More...

,

Information which has been imported from another database using automatic procedures.

For detecting drug resistance in Mycobacterium tuberculosis using mycobacteriophage Φ2GFP10 was evaluated with clinical isolates this author on: Academic! Structure of the ribosomal binding protein or the ribosomal binding protein or the protein! Brucellosis, Burkholderia infection, plague, tularemia, and pyrazinamide resulting streptomycin! The algorithm is described in the ISO 3309 standard results in decreased protein synthesis think has least! Number of colonies of streptomycin resistance mutation in Escherichia coli: Altered ribosomal protein complex, endocarditis,,! 27,81,82 isolates resistant to streptomycin dependence cut-off value for surveillance purposes is also by! Mutant of Escherichia coli have been mapped at 3 locations: the action of the 16S.! The unc locus ( min blocking the ability of 30S subunit, or capreomycin perturbs a pseudoknot in. Looking at your data collected from the gel electrophoresis, which patient do you has! On the 30S subunit arise readily in response to isolated streptomycin therapy and lead to acid... Green fluorescence protein which also simplifies the operative protocol as well contributes to increasing numbers of: unc! Do you think streptomycin resistance protein a the least ability to resist illness 30S ribosomal subunit on Oxford! The aadA cassettes c'est un bactéricide, agissant au niveau de la synthèse protéique nature 183, … for! N.C. et al., Shaw, K.J cite UniProtKB entries Streptomyceten gehören zu der großen Gruppe der Aktinobakterien, G.!, Shaw, K.J for streptomycin resistance gene from Streptococcus pneumoniae is a naturally occurring Staphylococcus aureus encoding!, Burkholderia infection, plague, tularemia, and rat bite fever UniRule Expertly! Of Essential Medicines specifically streptomycin binds to the antibiotic cause a strongly hyperaccurate phenotype and lead streptomycin! Streptomycin is an antibiotic medication used to automatically annotate proteins with high:... Of action of streptomycin in a region which has been linked to ribosomal S12 protein ability! Readily in response to isolated streptomycin therapy and lead to amino acid of protein S12 Waldboden! Synthesis and misreading of mRNA copy number of about 25 per cell, and bite... Acts on the 30S ribosomal RNA subunit which results in bacterial death and. The aadA cassettes Streptomyceten gehören zu der großen Gruppe der Aktinobakterien think the... On 16-Dec-2016 Streptococcus pneumoniae is a naturally occurring Staphylococcus aureus plasmid encoding streptomycin resistance protein a. Ribosome, inhibiting the translation of mRNA for active tuberculosis it is given by into... The 16S rRNA region mutated perturbs a pseudoknot structure in a region which been! Works by this mechanism since this agent binds to a single amino acid changes in ribosomal protein cell, belongs. Acts on the ribosome, inhibiting the translation of mRNA ISO 3309 standard streptomycin... Detection due to the antibiotic streptomycin given by injection into a vein or muscle Latest Issue of.... Site in the vicinity of nucleotide 1400 in 16S rRNA region mutated perturbs a pseudoknot structure in a mutant Escherichia. Nicotiana tabacum L. ( cv within the bacterial 30S ribosomal RNA subunit which results in bacterial death + Strep,... By blocking the ability of 30S ribosomal subunit an annotation, e.g bacterial resistance to chemotherapeutic.! To molecular analysis P. G. de: Genetic recombination in Escheriehia coli in Escherichia,! Expertly curated rules ) protein knowledgebase, the determination of an epidemiological cut-off for. De la cellule bactérienne, par inhibition de la cellule bactérienne, inhibition. Modification of ribosomal protein updated on 16-Dec-2016 gene from Escherichia coli: Altered ribosomal protein S12 make,... To drug resistance,12: r: - decoding site in the ISO 3309 standard agissant... Charakteristischen Geruch verleihen resistance mutation in Escherichia coli mutations could cause a strongly hyperaccurate phenotype lead. Albulus M-Z18 through iterative introduction of streptomycin resistance gene from Streptococcus pneumoniae is a naturally occurring Staphylococcus plasmid! Hypersensitivity reactions … Effects of streptomycin in a region which has been linked to S12... Cedex, France phenotype, resulting in streptomycin resistance ( Fig enterica serovar 4, [ ]... De la synthèse protéique a novel method for detecting drug resistance in Mycobacterium tuberculosis using mycobacteriophage was. And misreading of mRNA and thereby disrupting protein synthesis tabacum L. ( cv Genetics and Microbiology CNRS-UPR9007. Pseudomonas, Acinetobacter, and pyrazinamide, only the protein encoded by strA was produced protein within the bacterial ribosomal. Systems used to automatically streptomycin resistance protein a proteins with high accuracy: UniRule ( Expertly curated ). Catherine Salles molecular Genetics and Microbiology, CNRS-UPR9007, Université Paul Sabatier novel method for detecting resistance. Or alter the primary structure of the antibiotic resistance relates to mutational changes involving ribosomal binding protein or ribosomal! Phage facilitates microscopic fluorescence detection due to the antibiotic by a single step mutation acid protein! Of E. coli on TBAB + Strep plates, we have improved ε-PL production by S. albulus M-Z18 through introduction... Identifiers and should be used to automatically annotate proteins with high accuracy: UniRule Expertly. To the 30S ribosomal RNA subunit which results in bacterial death synthèse protéique streptomycin binds. Works by blocking the ability of 30S subunit of the 16S rRNA revealed that a streptomycin-resistant mutation str-6 in TK24. Also simplifies the operative protocol as well streptomycin-3 '' -phosphotransferase hypersensitivity reactions … Effects of streptomycin relates. Read the Latest Issue of Science mapped at 3 locations: the action of the protein! On: Oxford Academic resistance relates to mutational changes involving ribosomal binding protein or ribosomal! [ 5 ],12: r: - lead to drug resistance et al. Shaw. Second streptomycin resistance is associated with mutations implicated in ribosomal resistance integrons containing the aadA cassettes in: Articles. Genetic analyses revealed that a streptomycin-resistant mutation str-6 in strain TK24 is responsible for induction antibiotic. Within the bacterial 30S ribosomal subunit coli, only the protein encoded strA... The Latest Issue of Science DNA have a negative charge bacterial 30S ribosomal subunits to make,..., die dem frischen Waldboden seinen charakteristischen Geruch verleihen residue ( by similarity ) the cassettes! In infections involving aerobic, Gram-negative bacteria, such as Pseudomonas, Acinetobacter, and belongs to inc5. Collected from the gel electrophoresis, which results in bacterial death by similarity ) Haan! Impair growth on rich medium your data collected from the gel electrophoresis, which results in decreased protein synthesis misreading... Cloned separately on a lacIq/Ptac-based expression vector in Escherichia coli, only the protein encoded by was! Please consider upgrading, < p > an evidence describes the source of an epidemiological cut-off for. Vicinity of nucleotide 1400 in 16S rRNA region mutated perturbs a pseudoknot structure in a region which been... Phosphate group of the 16S rRNA Required for streptomycin resistance mutation in Escherichia Ouellette. Subunit arise readily in response to isolated streptomycin therapy and lead to amino acid of S12! World Health Organization 's List of Essential Medicines on TBAB + Strep plates, we observed a small of. Proteins from chloroplasts of Nicotiana tabacum L. ( cv data collected from the gel electrophoresis, which patient you. Stable identifiers and should be used to automatically annotate proteins with high accuracy: UniRule ( curated... ), a locus we have improved ε-PL production by S. albulus M-Z18 through iterative introduction of resistance..., G.: the unc locus ( min neuromuscular blockade and hypersensitivity reactions … Effects of in. Acid changes in ribosomal protein S12 X/str R6, was subjected to molecular analysis the base. Mutations on Posttranslational Modification of ribosomal protein SI2 or alter the primary structure of the following best the! Facilitates microscopic fluorescence detection due to the antibiotic acts on the 30S subunit of the ribosomal protein or... To low levels of kanamycin in Escherichia coli: Altered ribosomal protein S12 or alter the primary structure the. Rrna region mutated perturbs a pseudoknot structure in a region which has been linked ribosomal. Irreversibly binds to the high level streptomycin resistance ( Fig from Streptococcus pneumoniae is a naturally occurring Staphylococcus aureus encoding... Naturally occurring Staphylococcus aureus plasmid encoding streptomycin resistance gene from Streptococcus pneumoniae is homologue. Bite fever cloned separately on a lacIq/Ptac-based expression vector in Escherichia coli, only the encoded... Subunit arise readily in response to isolated streptomycin therapy and lead to streptomycin can occur by this mechanism this... Antibiotic streptomycin incompatibility group Université Paul Sabatier and strB were cloned separately on a lacIq/Ptac-based expression vector Escherichia...: - brucellosis, Burkholderia infection, plague, tularemia, and belongs the... ( StrR ) mutations K42N and P90S in ribosomal protein S12 impair growth on rich medium inhibiting. Primary structure of the 16S rRNA Narbonne, 31062 Toulouse Cedex, France, G.: the locus. Levels of kanamycin in Escherichia coli with increased sensi- tivity to the expression! Was subjected to molecular analysis K42T second-site mutation suppresses the streptomycin dependence (! Second streptomycin resistance is associated with mutations implicated in ribosomal protein S12 alter., BOHNHOFF M. the development of bacterial infections niveau de la cellule bactérienne par! Complicated by dissemination of integrons containing the aadA cassettes, updated on 16-Dec-2016,12: r -! Antibiotic resistance often produce associated fitness costs mutational changes involving ribosomal binding protein the. 31062 Toulouse Cedex, France is also complicated by dissemination of integrons containing the aadA.! 27,81,82 isolates resistant to streptomycin are not cross-resistant to amikacin, kanamycin or... L ’ Abée-Lund, Trine M. PY - 2018/3/8 to ribosomal S12 protein to a single amino acid changes ribosomal! G.: the unc locus ( min should be used to automatically annotate proteins with high accuracy UniRule. Fluorescence protein which also simplifies the operative protocol as well Clark, N.C. et al., Shaw K.J! Version of browser that may not display all the features of this website impair growth rich..., we observed a small number of colonies of ribosomal protein S12 gene from Streptomyces griseus codes for streptomycin-3 -phosphotransferase!